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picrg entry vectors  (Addgene inc)


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    Addgene inc picrg entry vectors
    Picrg Entry Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/picrg+entry+vectors/piCRg+Entry+(Plasmid+%2358904)/bio_rxiv__64898__2026__03__09__710586-222-19-22
    Average 86 stars, based on 3 article reviews
    picrg entry vectors - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    In Vitro:

    Article Title: A Genetically Engineered Human Organoid Model Reveals Distinct Genetic and Epigenetic Barriers of Lineage Plasticity in Early PDAC Transformation
    Article Snippet: .. To generate SMAD4 sgRNA in vitro , a T7 promoter was added to sgRNA templates by PCR amplification on piCRg Entry vectors (Addgene #58904) using CRISPR-specific forward primers and a universal reverse primer gRNA-R (5’-AAAAGCACCGACTCGGTGCC-3’). .. T7-sgRNA PCR products were used as templates for in vitro transcription using the MEGAshortscript T7 kit (Life Technologies, AM1354).

    Polymerase Chain Reaction:

    Article Title: A Genetically Engineered Human Organoid Model Reveals Distinct Genetic and Epigenetic Barriers of Lineage Plasticity in Early PDAC Transformation
    Article Snippet: .. To generate SMAD4 sgRNA in vitro , a T7 promoter was added to sgRNA templates by PCR amplification on piCRg Entry vectors (Addgene #58904) using CRISPR-specific forward primers and a universal reverse primer gRNA-R (5’-AAAAGCACCGACTCGGTGCC-3’). .. T7-sgRNA PCR products were used as templates for in vitro transcription using the MEGAshortscript T7 kit (Life Technologies, AM1354).

    Amplification:

    Article Title: A Genetically Engineered Human Organoid Model Reveals Distinct Genetic and Epigenetic Barriers of Lineage Plasticity in Early PDAC Transformation
    Article Snippet: .. To generate SMAD4 sgRNA in vitro , a T7 promoter was added to sgRNA templates by PCR amplification on piCRg Entry vectors (Addgene #58904) using CRISPR-specific forward primers and a universal reverse primer gRNA-R (5’-AAAAGCACCGACTCGGTGCC-3’). .. T7-sgRNA PCR products were used as templates for in vitro transcription using the MEGAshortscript T7 kit (Life Technologies, AM1354).

    CRISPR:

    Article Title: A Genetically Engineered Human Organoid Model Reveals Distinct Genetic and Epigenetic Barriers of Lineage Plasticity in Early PDAC Transformation
    Article Snippet: .. To generate SMAD4 sgRNA in vitro , a T7 promoter was added to sgRNA templates by PCR amplification on piCRg Entry vectors (Addgene #58904) using CRISPR-specific forward primers and a universal reverse primer gRNA-R (5’-AAAAGCACCGACTCGGTGCC-3’). .. T7-sgRNA PCR products were used as templates for in vitro transcription using the MEGAshortscript T7 kit (Life Technologies, AM1354).



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